Vacuum Manifold

Products - Microarray Hardware - Vacuum Manifold

TeleChem’s ArrayIt® brand High-Capacity Vacuum Manifold (AVM) provides an extremely convenient and high-throughput device for manual and semi-automated vacuum purification of PCR products, dye terminator products, fluorescent probes, and other types of molecules used in microarray analysis.

Table of Contents

Introduction
Congratulations on taking a big step towards improving the economies of scale, quality and speed of your genomics research. This booklet contains information needed to use TeleChem’s ArrayIt^® brand High-Capacity Vacuum Manifold (AVM).

Quality Control
TeleChem assures the performance of this product. The finest scientific research went into the development of this product. Rigorous quality control monitoring of every device guarantees that this vacuum manifold exceeds the highest industry standards.

Product Description
TeleChem’s ArrayIt® brand High-Capacity Vacuum Manifold (AVM) sets a new standard for laboratory vacuum manifolds. The AVM is easy to use, rugged, durable, and can be used with any standard footprint microplate.

Users will appreciate the following features:

Compatible with all standard microplates
Accommodates 96- and 384-well plates
Clear acrylic top allows easy visibility
Ideal for PCR product, dye terminator and fluorescent probe purification
Filter 4 plates simultaneously
Can be operated manually or in a semi-automated manner
Central reagent tray for easy liquid transfer
Eluent reservoir holds flow through
Dual valves for one or two vacuum lines
Can be used with any vacuum pump
Small footprint
Provides years of maintenance-free operation

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com (under the subject heading please type ArrayIt technical assistance). By telephone: (408) 744-1331, Monday—Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!

Short Protocol

Remove Vacuum Manifold from packaging material.
Connect vacuum spindle to vacuum pump.
Place 96-well or 384-well plates into position of 4 filter ports.
Pipette samples in filter plates.
Turn valve to “on” position to initiate vacuum filtration.
Empty eluent reservoir after use.
Rinse and wipe dry for storage.

Complete Protocol

Remove Vacuum Manifold from packaging material. Place the Vacuum Manifold on a bench top or other solid working surface.
Connect vacuum spindle to vacuum pump. Turn the vacuum valve into the “off” position, such that the red valve is perpendicular to the direction of airflow. Attach a vacuum pump to the Vacuum Manifold by inserting the vacuum tubing over the threaded vacuum spindle. Power the Vacuum Pump on.
Place 96-well or 384-well plates into position of 4 filter ports. The 4 filter ports on the top of the Vacuum Manifold hold up to 4 standard microplates. The Vacuum Manifold is ideally suited for use with TeleChem’s PCR Purification Kits, Dye Terminator Clean-Up Kits, and Fluorescent Probe Purification Kits. Empty filter port locations can be fitted with empty plates in experiments using fewer than 4 microplates. The Vacuum Manifold is well suited for all vacuum-based filtration procedures.
Pipette samples in filter plates. Use a manual or automatic pipetting device to transfer buffers and samples into the microplates. The reagent reservoir on the top center portion of the vacuum manifold can be used to hold buffers during the pipetting steps.
Turn valve to “on” position to initiate vacuum filtration. Once the samples have been transferred to the microplates, initiate filtration by turning the red vacuum valve one-quarter turn clockwise into the “on” position. Samples should flow slowly through the filter plates and into the eluent reservoir. If a vacuum seal is not achieved, make certain that all 4 microplates are seated correctly against the black rubber gaskets in the filter ports. Vacuum flow can be turned on and off during multiple pipetting steps, by turning the vacuum valve into the open and closed positions. At the end of the filtration process, turn the valve into the “off” position.
Empty eluent reservoir after use. Sample eluent will accumulate in the white eluent reservoir located between the top and bottom portions of the Vacuum Manifold. Eluent is removed by inverting the reservoir into a sink or waste container.
Rinse and wipe dry for storage. After use, make certain to rinse and clean the Vacuum Manifold in preparation for future use.

Fig. 1. ArrayIt® brand High-Capacity Vacuum Manifold (AVM) shown in disassembled form. The base, eluent reservoir and clean top fit together into a compact assembly. Vacuum filtration of PCR products, dye terminator products, fluorescent probes, and other molecules is highly recommended for microarray and DNA sequencing applications

Equipment and Reagents

Vacuum Pump

PCR Purification Kits

Fluorescent Probe Purification Kits

Dye Terminator Cleanup Kits

Cleanroom Wipes

Troubleshooting Tips

Manifold fails to pull sufficient vacuum

Make certain all 4 microplate ports are sealed
Make sure manifold top and base are sealed

Ordering Information

Product

Description

Catalog I.D.

Price per manifold (U.S. dollars)

ArrayIt® brand High-Capacity Vacuum Manifold

High capacity vacuum manifold for use with PCR, dye terminator, fluorescent probe purification, or any other microplate-based filtration application

AVM

$1,400
click here to purchase online

*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.

To order ArrayIt® Brand Products: call 408-744-1331, FAX 408-744-1711 or click on the button below to go directly to the purchase page.

Operation Conditions
TeleChem’s ArrayIt® brand Vacuum Manifold (AVM) should be stored and operated at ambient temperatures and humidity. The vacuum manifold performs well across a wide range of ambient temperatures and relative humidity and is compatible with class 100 cleanrooms. It should provide many years of maintenance-free use if operated properly.

Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes only. ArrayIt® brand products are not for use in human diagnostics or for drug purposes, nor are they to be administered to humans in any way. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® brand products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with our ArrayIt® brand product should be reported to TeleChem immediately. TeleChem’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product including deviations from our protocols is the full responsibility of the user, and TeleChem makes no warranty or guarantee under such circumstances.