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Products - Microarray Instruments - Microarray Imprinter
Stop using a messy pap pen! The Microarray Slide Imprinter is an easy and cost effective way to partition microarray slides and substrates with a hydrophobic pattern for microarray and other slide based applications. This durable instrument is ideal for high throughput microarray experiments where multiple assays need to be run on a single microarray slide/substrate with minimal risk of contamination between wells.
The Microarray Slide Imprinter is unique in substrate slide partitioning technology as it empowers the researcher to partition any microarray substrate substrate slide in the laboratory.
Gaskets and other approaches:
Gaskets without adhesive may not seal completely if the hardware is not designed properly and over time gaskets can wear out. If you prefer a high quality gasket type system, click here. Gaskets with adhesive can leak if the adhesive is soluble in the solvents used in processing the microarray. Gaskets must be removed before scanning which can cause some types of microarray surface chemistries to peel off the surface. Hydrophobic markers such as PAP pens are tedious and difficult to apply, are soluble in certain solvents, and the resulting partitions are often inaccurate. Some manufactured barriers are expensive, must be ordered in quantity, and design changes can be difficult and time consuming to implement.
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Microarrays per substrate |
Reaction volume per microarray |
Estimated number of microarray spots per well |
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12 |
40 µl |
500 |
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16 |
60 µl |
750 |
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24 |
60 µl |
500 |
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48 |
6-7 µl |
48 |
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192 |
1-1.5 µl |
16 |
End users will appreciate the following:
- imprint hydrophobic patterns onto any slide and surface (any design/pattern can be imprinted)
- empowers high throughput sample processing
- pays for itself quickly by amortizing surface chemistry cost over many microarray experiments on a single slide/substrate
- patterns meet micro spotting pin spacing of all Stealth and 946 printheads and microarrayer programming
- parallel loading of samples using multi-channel pipettes or automated pipetting liquid systems such as Oasis and others
- does not contribute to background or light scattering in scanning for colorimetric, CCD and laser/pmt fluorescent microarray scanners
- stays on throughout all microarray processing and detection steps
- compatible with a wide variety of aqueous and solvent based buffers
- works for both DNA, protein, carbohydrate, small molecule and other types of microarrays
- easy to use, no setup or assembly required
- fast, each slide only takes a few seconds to imprint
- fun to use
- works with any and all microarray surface chemistry including hydrogels and membrane based surfaces such as SuperNitro and SuperProtein
- inexpensive, low cost to operate and own
How the Microarray Slide Imprinter works:
The Microarray Slide Imprinter provides a fast and convenient way to define multiple “wells” on a single microscope slide size substrate to create separated regions to process many assays on a single substrate. This is accomplished by imprinting a thin, inert, hydrophobic film patterned onto the surface of a slide/substrate through the use of a pre-designed “stamp.”
Step 1. Select stamp and make sure it is immersed in a bath of hydrophobic film polymer.
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Step 2. A microscope slide size substrate is inserted into a cartridge above the bath and held accurately for consistent positioning. Microarray locations are consistent from slide to slide.
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Step 3. A lever on the outside of the imprinter is pulled so that the “stamp” can touch off on the surface of the substrate, thus imprinting the desired hydrophobic barrier lines onto the substrate. Microarrays can be now be manufactured and processed.
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Since the printed hydrophobic pattern printed is thin, the imprinted slides/substrates can be inserted directly into microarrayers and microarray processing racks and wash stations and directly into a microarray scanners without any further modifications. The coating is highly hydrophobic, does not dissolve in most solvent systems, and will stay on the slide through multiple wash steps and overnight incubations. The Microarray Slide Imprinter offers excellent flexibility. The imprinted hydrophobic barriers can be made in any design and works with all microarray surface chemistries including membranes and hydrogels. Additionally the barrier shows excellent stability in most solvents through repeated washes, is chemically inert and does not contribute to light scatter or background noise in detection.
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Background light scattering comparison between imprinted microarray slide (left image) and a slide with common hydrophobic ink (right image) scanned with a typical laser/pmt microarray scanner. Yellow border added for visualization of data. Note that the imprinted pattern on the slide (left image) does not show up in the scan and therefore does not contribute to background noise nor does it scatter any signal. Border on the ink printed slide shows completely saturated signals (right image).
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Ordering Information
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Description |
Catalog ID |
Price (US dollars)* |
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12 Microarrays |
MSI12 |
$2,995 |
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16 Microarrays |
MSI16 |
$2,995 |
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48 Microarrays |
MSI48 |
$2,995 |
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192 Microarrays |
MSI192 |
$2,995 |
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Custom Design |
MSICUST |
$3,495 |
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Hydrophobic polymer |
DNAWAX PROWAX |
$55 |
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12 Microarrays |
STAMP12 |
$595 |
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16 Microarrays |
STAMP16 |
$595 |
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48 Microarrays |
STAMP48 |
$595 |
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192 Microarrays |
STAMP192 |
$595 |
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Replacement Stamps. Please specify number of wells per substrate. All stamps are interchangeable. |
Inquire |
Inquire |
*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.
Warranty
ArrayIt® brand products have been scientifically developed and are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All ArrayIt® products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with an ArrayIt® brand product should be reported to TeleChem immediately. TeleChem’s liability is limited to the replacement of the product, or a full refund. Any misuse of this product including deviations from our protocols is the full responsibility of the user, and TeleChem makes no warranty or guarantee under such circumstances.
For a downloadable version of this protocol, please click here.
Copyright 1993-2008 TeleChem International, Inc. All rights reserved.
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