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Products - Amplification & Labeling - Reference mRNAs & Labeled cDNA Mixtures for DNA Microarray Applications in Gene Expression Profiling, SNP Analysis and Genomics
Universal Reference Human mRNA is a proprietary blend of amplified high-quality mRNA purified from a diverse set of human, mouse or rat tissues. This reference mRNA provides a common denominator for accurate and reproducible comparisons of gene expression data. Pooled reference mRNA can be used for multicolor hybridization experiments using DNA microarrays. The signal generated from this mRNA will ease the normalization process, in both intensity-based and reference-channel-based normalization. The use of pooled reference RNA has been shown to reduce inter and intra-laboratory variations of microarray data. This product is compatible with all commercially available microarray labeling systems as well as other research applications such as RT-PCR, and QRT-PCR that demand high quality reference pool of mRNA. New Universal Reference Cy3- and Cy5-labeled cDNA mixtures offer exceptional normalization standards for two-color hybridization experiments.
Reference mRNA can also be delivered as indirectly labeled cDNA (see ordering information).
Can be used stand alone or in conjunction with:
ArrayIt Universal Reference mRNA:
Differences between using total RNA and mRNA in labeling for microarrays are as follows:
1. When labeling using total RNA in either direct or in-direct methods, oligo dT to prime the RT reaction is used. Therefore the labeling is limited to the capability of the reverse transcriptase to generate large fragments on a single run. On the other hand, when using mRNA, a mixture of oligo dT and random primers is used, allowing a much better cDNA:dye ratio and much better labeling. For this reason, many microarray users prefer to purify mRNA prior to reverse transcription and labeling.
2. The use of reference mRNA becomes absolutely necessary and saves a lot of time and money, when a user is working from small quantities of RNA and is performing mRNA amplification for microarray studies. The amplified mRNA from the samples can be used directly with the reference mRNA, saving one round of amplification. Since, the sample total RNA (purified by the user) and the reference total RNA (purified by the vendor) will have different purity, the results will vary in the amplification reaction as well.
Number of labeling per pack of 30 µg
For direct labeling use 2-3 µg/reaction, for indirect labeling use 1-2 µg/reaction.
Figure 1. Quality testing of Human Universal mRNA
*Add shipping and handling to all orders.
*International pricing may vary as much as 30% (or more depending on country) due to import duties, stocking fees and technical support.
Arrayit reference mRNAs should be stored at –20°C or -80°C.
Arrayit Universal mRNA products are sold for research purposes only. Arrayit products are not for use in human diagnostics or for drug purposes, nor are they to be administered to humans in any way. Extreme care and exact attention should be practiced in the use of the materials described herein. Arrayit products are subject to extensive quality control and are guaranteed to perform as described when used properly. This product is stable for at least 1 year from the date of purchase when stored at –80°C. Any problems with these products should be reported to Arrayit immediately. Our liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and Arrayit makes no warranty or guarantee under such circumstances.
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