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Arrayit Corporation (ARYC) leads and empowers the genetic, research, pharmaceutical, and diagnostic communities through the discovery, development and manufacture of proprietary life science technologies and consumables for disease prevention, treatment and cure.  - Powerful Science for Life™

Products - Microarrayers - SpotBot® 4 Desktop Personal, SpotBot® 4 Protein Edition and SpotBot® 4 HotBot Edition Microarrayers

SpotBot4
New!! Arrayit SpotBot® 4 Personal Microarrayers provide the market's most advanced personal microarrayers, featuring our patented contact printing technology installed in more than 4,200 laboratories worldwide. SpotBot® 4 Microarrayers empower DNA, oligonucleotide, amplicon, BAC, antibody, antigen, peptide, carbohydrate, small molecule, lipid, cell extract and whole cell microarray manufacturing in minutes on your desktop for genotyping, gene expression, protein expression profiling, protein binding, and myriad other microarray applications. Large OEM arrangements permit the integration of your microarray manufacturing application into our time-tested personal microarray platform. SpotBot® 4 systems include a BioBlue personal computer, LED display, keyboard, mouse, mouse pad, cables and accessories for easy installation.

SpotBot® 4 System Specifications and Features

  • SpotBot® 4 instruments represent a significant advance over earlier SpotBot® 1-3
  • Extra heavy duty gantry offers improved precision, speed and durability
  • Arrayit patented contact printing technology (U.S. 6,101,946) adds confidence
  • Vision System option allows high-resolution printing on custom substrates and devices
  • Professional series printhead permits precision pin guidance for precise rows and columns
  • Four printing pins configured in a 2 x 2 pattern at 4.5 mm spacing
  • Professional series printhead reduces friction and allows minimal force contact printing
  • Axis resolution and repeatability of ±10 µm
  • Deck accommodates 14 standard glass substrate slides (1 x 25 x 76 mm)
  • Deck accommodates 1 microplate (384-wells)
  • Complete deck and microplate cooling from ambient to 4°C for Protein Edition
  • Complete deck and microplate heating from ambient to 45°C for “HotBot”€ Edition
  • Humidity control option from 10-80% relative humidity (RH)
  • Megasonic Wash Station option enhances pin cleaning
  • Compatible with all professional, 946 and Stealth microarray printing pins
  • Printing routines allow 1-5 technical replicates per sample
  • 3,600 spots per sub-microarray (9 x 9 mm)
  • 50,400 spots per entire substrate (18 x 63 mm)
  • Printing time of 2 hrs per 384-well microplate
  • >1,000 samples in 6 hours
  • Runs on Microsoft Windows 7
  • Power requirements: Standard 120-volt or 220 volt outlets
  • Ease-of-operation via new version 4 Graphical User Interface (GUI)
  • SpotBot® 4 size (H x L x W): 29 cm (11.5 in) x 30 cm (11.8 in) x 30 cm (11.8 in)
  • SpotBot® 4 weight: 6.2 Kg (13.6 lbs)
  • Private branding, OEM agreements and large volume pricing available
  • World’s only truly portable desktop microarrayers
  • World’s only turnkey desktop protein microarray manufacturing systems
  • Create a miniature “cold room”€ť on your desktop with 4°C Protein Edition deck cooling option
  • Flow-through liquid cooled platen maximizes deck cooling speed, efficiency and uniformity
  • Systems shipped with factory calibration for ease of set-up and use
  • Micro-robotics enable precision motion control in a highly compact footprint
  • Near zero thermal emission, ultra-low energy consumption, and quiet operation
  • Rapid flow wash station and forced air pin drying enables efficient pin cleaning
  • Humidity control of 5% per minute and ±1 % at steady state
  • Minimal wash buffer consumption of <1.0 liter per 8 hours of operation
  • Minimal air turbulence virtually eliminates sample evaporation during printing
  • Vibration dampeners allow high printing quality in any laboratory
  • Class 1 cleanroom compatible
  • 400+ installations worldwide
  • World’s most popular personal microarrayer product line
  • Customer Installation time of 1 hour
  • Continuous on-line technical support via email and Skype

protein-microarrayer
Arrayit SpotBot® 4 Personal Microarrayer with Protein Edition option including custom thermal deck and chiller bath for 4°C to ambient temperature control ideal for protein and reverse phase microarrays. The “HotBot” option permits 4°C to 45°C temperature control for protein microarrays as well as enzyme and whole celll microarrays requiring heating to 37°C.

Table 1. SpotBot® 4 Personal Microarrayer System Components and Options.

System Component

Description

Standard

Option

SpotBot® 4 Personal Microarrayer

World’s most popular personal microarray printers featuring compact footprint, micro-robotic motion control, ±10 µm positional resolution, heavy duty gantry, patented contact printing technology, and environment enclosure for completely automated turnkey desktop microarray printing.

Yes

 

Contact printing technology

Arrayit patented contact printing technology covered by US 6,101,946 patent, installed in >4,500 laboratories worldwide, featuring microarray printing pins with defined uptake channel and flat tips for consistent high quality microarray printing.

Yes

 

Adjustable spot diameter

SpotBot® 4 professional series printhead will hold Arrayit’s entire line of Professional, 946 and Stealth microarray printing pins, allowing users to control printed spot diameter from 37.5-560 µm.

Yes

 

Instrument enclosure

SpotBot® 4 instruments include a transparent instrument case that protects against particulate contaminants and allows humidity and temperature control during operation.

Yes

 

Compact footprint

SpotBot® 4 instruments have a highly compact 0.03 cubic meter (1.0 cubic foot) instrument dimensions for market’s most compact microarray printer.

Yes

 

Low thermal emission

SpotBot® 4 instruments utilize advanced micromotors for low thermal emission during operation to ensure the integrity of biological molecules and surface chemistry.

Yes

 

Universal biochemistry platform

SpotBot® 4 patented contact printing technology allows printing of virtually any biomolecule including DNA, protein, small molecule, carbohydrate, lipid, cell extract, whole cells and many other sample types for ultimate flexibility.

Yes

 

Wide sample viscosity range

SpotBot® 4 patented contact printing pins load samples by simple capillary action, which enables the use water, glycerol, and DMSO and other light solvents to accommodate diverse sample types.

Yes

 

Low sample volumes

SpotBot® 4 instruments utilize standard footprint 384-well microplates to allow easy and convenient workflow from sample preparation to microarray printing. Recommended sample volumes are 4-8 µl per 384-well.

Yes

 

Highly affordable manufacturing

SpotBot® 4 patented contact printing pins load 0.25  µl per sample uptake from a recommended sample volume of 4-8 µl per 384-well to allow the use of small sample volumes for highly affordable manufacturing.

Yes

 

Gentle delivery mechanism

SpotBot® 4 patented contact printing pins print samples using a gentle surface tension “ink-stamping” method to ensure the integrity of microarray substrates including membrane coatings and maintain the activity of sensitive printed molecules including proteins and whole cells.

Yes

 

Standard glass substrate slide format

SpotBot® 4 instruments accommodate the industry standard “open platform” 1 x 25 x 76 mm glass substrate slide format for compatability with Arrayit’s complete microarray slide product line.

Yes

 

Supports many printing surfaces

SpotBot® 4 instruments and patented contact printing pins enable printing onto a wide range of different substrates including glass, plastic, silicone and other materials for manufacturing and innovation flexibility.

Yes

 

Rapid pin replacement

SpotBot® 4 patented contact printing technology allows customers to replace microarray printing pins in real time without any special tooling required to allow easy changes in spot diameter and loading of new pins in cases of pin clogging or damage.

Yes

 

110-Volt and 220-Volt System Components

SpotBot® 4 printers and accessories are compatible with both 110 and 220 volt electrical sources for domestic and international operation at customer locations worldwide.

Yes

 

Accessory Control Module

SpotBot® 4 printers utilize an Accessory Control Module (ACM) with power supply and anti-vibration feet to allow easy and convenient control of instrument peristaltic pump, dry station, humidity control and megasonic wash station.

Yes

 

946MP4 printing pins

Arrayit 946MP4 microarray printing pins, 4 each, featuring 0.25 µl sample channel, 100 µm wide tip and nominal 135 µm diameter printed microarray spots for a wide range of microarray manufacturing applications.

Yes

 

946 pin tool

Arrayit 946 pin tool (inserted in the hole in the pin collar) is used to remove 946MP4 and other 946 style microarray printing pins from the printhead to allow pin cleaning and loading different pin types.

Yes

 

Calibration test pin

SpotBot® 4 microarray printers include 1 calibration test pin (Cat. SSP015) with a solid 375 µm wide tip for testing new printing routines before running the instrument with the more expensive 4 x 946MP4 pins in the printhead.

Yes

 

Wash buffer container

SpotBot® 4 microarray printers include a portable 1 liter wash buffer container to allow customers to easily monitor and replace wash station wash buffer during instrument operation.

Yes

 

Wash waste container

SpotBot® 4 microarray printers include a portable 1 liter wash waste container (placed on the floor below the instrument)  to allow customers to easily collect and discard used wash buffer produced by the wash station during instrument operation.

Yes

 

SpotBot® 4 wash buffer

SpotBot® 4 microarray printers include 1 liter of 1X wash buffer to fill the wash buffer container prior to instrument operation. Wash buffer is pumped through the wash station by the ACM peristaltic pump and cleans the microarray printing pin tips during the printing process.

Yes

 

Substrate locator

SpotBot® 4 microarray printers include an “L-shaped” L-bracket to hold the 14 substrate slides (1 x 25 x 76 mm) on the deck of the instrument during the printing process. The substrate locator is placed on the right side of the instrument deck between the 2 x 7 array of substrate slides and the substrate locator tensioner spring.

Yes

 

Substrate slides

SpotBot® 4 microarray printers include one box of 25 SuperEpoxy 2 or SuperEpoxy 3 substrate slides (1 x 25 x 76 mm) to enable customers to print DNA and protein microarrays immediately upon setting up their instrument. Substrate slides are held in position on the deck using the substrate locator.

Yes

 

Anti-vibration pads

SpotBot® 4 microarray printers include 4 anti-vibration pads (placed under each of the 4 instrument feet) to absorb vibrations on the laboratory bench and improve the precision of the printing process.

Yes

 

384-well microplates

SpotBot® 4 microarray printers include two 384-well microplates (Cat. MMP384) placed on the left side of the instrument deck between the microplate tensioner spring and microplate locator. SpotBot® 4 printing pins (4 each) transfer samples from the microplate onto the substrate slides during the printing process.

Yes

 

Print buffer

SpotBot® 4 microarray printers include one 50 ml bottle of 2X Micro Spotting Solution Plus (Cat. MSP), which is mixed 1:1 volume to volume with DNA samples and transfered into the 384-well microplate for printing. One 50 ml bottle of 2X Protein Printing Buffer (Cat. PPB) is provided with Protein and “HotBot” Edition SpotBot® 4 systems.

Yes

 

Data cable

SpotBot® 4 microarray printers include one USB data cable to transmit instrument commands between the computer and instrument. The USB connects to the back of SpotBot® 4 instrument next to the power supply port.

Yes

 

Power supply

SpotBot® 4 microarray printers include a power supply and voltage-appropriate power to provide electrical power to the instrument during operation. The power supply converts 110 volt or 220 volt power into a lesser voltage to run the instrument.

Yes

 

Personal computer

SpotBot® 4 microarray printers include a BioBlue Mini Computer with pre-installed system software and calibration files, LED flat panel display, DVI cable, keyboard, mouse, mouse pad, wireless antenae, Internet cable and voltage-appropriate power cords.

Yes

 

Warranty

SpotBot® 4 microarray printers include a comprehensive 90-day warranty that covers all system components (except pins and printhead). Additional warranty coverage in one-year increments is available as an option.

Yes

 

Extended warranty

SpotBot® 4 microarray printers optional extended warranty comprehensive coverage on all system components (except pins and printhead). Extended warranty coverage purchased in one-year increments is recommended for most users.

 

Yes

Vision System

SpotBot® 4 microarray printers offer an optional advanced high resolution Vision System with high-resolution digital camera, tripod, LED illumination source, and custom software interface to view and record absolute positional coordinates on the printing deck with 10 µm spatial resolution for biochip, biosensor, microfluidic and microelectronic applications.

 

Yes

Megasonic Wash Station

SpotBot® 4 microarray printers offer an optional Megasonic Wash Station for enhanced pin cleaning. The Megasonic Wash Station option is included as standard with all Protein and “HotBot” Edition systems.

 

Yes

Humidity Control

SpotBot® 4 microarray printers offer optional Humidity Control for ambient to 80% relative humidification to reduce sample evaporation during printing and for operation in geographical regions with low humidity. The Humidity Control option is included as standard with all Protein and “HotBot” Edition systems.

 

Yes

Dehumidification

SpotBot® 4 microarray printers offer optional Dehumidification for ambient to 10% relative humidification for operation in geographical regions with high humidity. The Dehumidification option is included as standard with all Protein and “HotBot” Edition systems.

 

Yes

SpotBot® 4 Protein Edition

SpotBot® 4 Protein Edition Personal Microarrayers are designed for a wide range of protein, reverse phase and whole cell microarray applications. Protein Edition systems include all of the standard features listed above plus custom temperature-controllable instrument deck, chiller bath (0°C to ambient) with insulated tubing assembly, voltage-appropriate power cord, cooling liquid (3 liters), Megasonic Wash Station, Humidity Control and Dehumidification.

 

Yes

SpotBot® 4 HotBot Edition

SpotBot® 4 HotBot Edition Personal Microarrayers are designed for a wide range of protein, reverse phase, whole cell and enzymatic microarray applications. HotBot Edition systems include all of the standard features listed above plus custom temperature-controllable instrument deck, chiller and heater bath (0°C to 45°C) with insulated tubing assembly, voltage-appropriate power cord, cooling and heating liquid liquid (3 liters), Megasonic Wash Station, Humidity Control and Dehumidification.

 

Yes

Scientific Publications
Click on the links to find more than 300 scientific publications featuring Arrayit SpotBot® Personal Microarrayers.

personal_microarrayer
Figure 1. SpotBot® 4 Personal Microarrayer in the home€ť position. The printed circuit board (middle left) enables fully automated computer-control. The professional printhead (center) contains a calibration pin in the first of 4 pin apertures.

microarray-wash
Figure 2.  SpotBot® 4 wash station uses wash buffer from the ACM peristaltic pump to clean the 4 pins during printing. The optional megasonic wash station transmits megasonic waves through the wash buffer for enhanced pin cleaning. The dry ports to the right of the wash station use pressurized air from the ACM air compressor to dry the pins after the wash steps.

microarrayer-plate
Figure 3. SpotBot® 4 in the sample loading position. The professional series printhead and four 946MP4 microarray printing pins load the first group of 4 wells in the 384-well microplate. Each pin loads 0.25 µl of sample from each well containing a 5 µl total sample volume

SpotBot-microarray
Figure 4. SpotBot® 4 Protein Edition Microarrayer printing dilutions of ArrayIt® Green540 and Red640 fluorescent dyes with four 946MP4 pins and a professional series printhead. Samples were printed in quintuplicate at 200 µm spot spacing. The uniformity of the rows and columns of printed spots reveals the excellent printing performance of the SpotBot® 4. Space bar = 500 µm.

microarray-tests
Figure 5. SpotBot® 4 Personal Microarrayer printing multiple microarrays per substrate slide using one 946MP4 microarray printing pin at 175 µm spot spacing printing on SuperAmine (Cat. SMM) microarray substrate slides. A total of 24 microarrays per substrate were printed using the Multiple Microarray Format SpoCLe Generator version 1.1.02 downloadable at http://spotsupport.com.

microarray_testing
Figure 6.  SpotBot® 4 Personal Microarrayer Multiple Microarray Format SpoCLe Generator version 1.1.02 allows the printing of multiple microarrays per substrate. The user selects the well pattern (number of microarrays), spot spacing in microns, print offsets in millimeters, and subgrid dimensions as columns and rows. The software then generates a graphic showing how the printed microarrays (orange icons) will appear on the substrate slides. The Multiple Microarray Format SpoCLe Generator version 1.1.02 is downloadable at http://spotsupport.com.

microarray-surface-tension
Figure 7. SpotBot® 4 Personal Microarrayer professional series printhead and four 946MP4 microarray printing pins shown in the printing position on the instrument deck. An overtravel distance of 0.5 mm is used to ensure efficient printing onto each glass substrate slide. The SpotBot® 4 Protein Edition option allows deck cooling from ambient to 4°C to enhance the stability of printed proteins, reverse phase protein microarray (RPPA) extracts, and other sensitive samples. The SpotBot® 4 HotBot Edition option allows deck cooling and heating from 4°C to 45°C to enhance the stability of printed proteins, reverse phase protein microarray (RPPA) extracts, and enable enzymatic and whole cell applications requiring 37°C deck heating.

Microplate_Printing
Figure 8. SpotBot® 4 printing into a 96-well glass bottom microplate using software downloadable at www.spotsupport.com. Microplate printing requires the microplate L-bracket locator (Cat. MLS) to hold the microplate and two pre-print substrate slides in postion during printing. System calibration is required prior to initiating microplate printing. Please do not attempt microplate printing without system calibration.

Arrayit-microarrayer
Figure 9. SpotBot® 4 Personal Microarrayer with a pofessional series printhead and four 946MP4 microarray printing pins in the safe Z position over the glass substrate slides. The substrate locator L-bracket immobilizes the substrate slides on the platen during printing using the substrate tensioner spring (middle right).

microarray-vision
Figure 10. SpotBot® 4 Personal Microarrayer Vision System with high-resolution digital camera, tripod, and custom software interface to view and record absolute positional coordinates on the printing deck. The Vision System allows visualization of microscopic substrate elements such as 100 µm microelectrodes (see insert) using a digital camera with 10 µm spatial resolution. This option is highly recommended for aerospace, bio-engineering and material science applications.

microarrayer-ACM
Figure 11. SpotBot® 4 Personal Microarrayer Accessory Control Module (ACM) including connections from left-to-right for the megasonic wash station, humidty control, peristaltic pump, pin drying station, power switch and ACM power supply (rear left). The megasonic cable should be connected to the threaded connector on the wash station and the peristaltic and dry station connections are color-coded as follows: peristaltic wash buffer inward flow to 1 liter wash buffer reservoir (green-green), peristaltic wash buffer inward flow to wash station inward flow (white-white), peristaltic wash waste outward flow to wash station outward flow (blue-blue), peristaltic wash waste outward flow to 1 liter wash waste container (yellow-yellow), and dry station forced air stream to dry station pin drying port (red-red). ACM anti-vibration pads should be placed under the 4 ACM feet as shown. Connect all ACM connections to the SpotBot® 4 prior to powering on the ACM and power off the ACM after each use. The SpotBot® 4 software should be launched prior to powering on the ACM to prevent software complications arising from power surges.

Ordering Information

Product

Description

Catalog ID

Price USD*

SpotBot® 4 Personal Microarrayer

SpotBot® 4 Personal Microarrayer featuring world famous Arrayit microarray technology, compact footprint, micro-robotic motion control, ±10 µm positional resolution, heavy duty gantry, patented contact printing technology, and environment enclosure for completely automated turnkey desktop microarray printing. Systems include patented contact printing technology, peripherals, consumables, personal computer and LED monitor and a 90-day comprehensive warranty.

SPA4

22,945
Purchase1

SpotBot® 4 Personal Microarrayer Protein Edition

SpotBot® 4 Personal Microarrayer Protein Edition featuring world famous Arrayit microarray technology, compact footprint, micro-robotic motion control, ±10 µm positional resolution, heavy duty gantry, patented contact printing technology, and environment enclosure for completely automated turnkey desktop microarray printing. Systems include patented contact printing technology, peripherals, consumables, personal computer and LED monitor and a 90-day comprehensive warranty. Protein Edition systems include a SpotBot® 4 Protein Microarrayer with liquid cooled deck, digital Peltier chiller bath to allow instrument deck cooling from ambient to 4°C, Accessory Control Module (ACM) box, humidity and dehumidification control, SuperEpoxy 3 Substrate Slides, reagents and consumables for protein printing, BioBlue personal computer, LED display, keyboard, mouse, mouse pad, cables and accessories for easy installation.

SPA4PRO

33,513
Purchase1

SpotBot® 4 Personal Microarrayer HotBot Edition

SpotBot® 4 Personal Microarrayer HotBot Edition featuring world famous Arrayit microarray technology, compact footprint, micro-robotic motion control, ±10 µm positional resolution, heavy duty gantry, patented contact printing technology, and environment enclosure for completely automated turnkey desktop microarray printing. Systems include patented contact printing technology, peripherals, consumables, personal computer and LED monitor and a 90-day comprehensive warranty. SpotBot® 4 HotBot Edition systems include a SpotBot® 4 HotBot Microarrayer with liquid heated and cooled deck, digital Peltier chiller and heating bath to allow instrument deck cooling and heating from 4°C to 45°C, Accessory Control Module (ACM) box, humidity and dehumidification control, SuperEpoxy 3 Substrate Slides, reagents and consumables for protein, enzyme and whole cell printing, BioBlue personal computer, LED display, keyboard, mouse, mouse pad, cables and accessories for easy installation

SPA4HOT

34,513
Purchase1

SpotBot® 4 Personal Microarrayer Vision System Option

Arrayit SpotBot® 4 Personal Microarrayers Vision System provide the market's most advanced personal microarrayers for microprocessors, microfluidics and other printing applications that require absolute location printing, featuring our patented contact printing technology installed in more than 4,200 laboratories worldwide. The SpotBot® 4 Vision System option includes high-resolution digital camera, tripod, LED illumination source, and custom software interface to view and record absolute positional coordinates on the printing deck, 10 µm spatial resolution highly recommended for R&D users printing on microarray, biochip, biosensor, microfluidic and microelectronic substrates for biochemistry, aerospace, bio-engineering, and material science applications.

SPA4V

9,360
Purchase1

SpotBot® 4 Personal Microarrayer Humidity Option

Arrayit SpotBot® 4 Personal Microarrayers Humidity Option provide the market's most advanced personal microarrayers with humidity control, featuring our patented contact printing technology installed in more than 4,200 laboratories worldwide. The SpotBot® 4 Humidity Option includes an Accessory Control Module with an extra air compressor, 1L humidification reservoir, control valve and tubing to connect to the back of the SpotBot® 4 Personal Microarrayer to control humidity from ambient to 80% RH.

SPA4H

1,679
Purchase1

SpotBot® 4 Personal Microarrayer Megasonic Option

Arrayit SpotBot® 4 Personal Microarrayers Megasonic Option provide the market's most advanced personal microarrayers with advanced megasonic pin cleaning wash station, featuring our patented contact printing technology installed in more than 4,200 laboratories worldwide. The Megasonic Option includes a megasonic controller added to the Accessory Control Module (ACM), megasonic connecting wire to connect the ACM to a fitting on the SpotBot® 4 instrument wash station containing a megasonic transducer disc to increase the efficiency of pin cleaning for protein microarray and other demanding applications.

SPA4M

3,303
Purchase1

SpotBot® 4 Personal Microarrayer Warranty Option

Arrayit SpotBot® 4 Personal Microarrayers Warranty Option provides the market's most advanced personal microarrayers warranty coverage including comprehensive warranties for our SpotBot® 4, SpotBot® 4 Protein Edition and SpotBot® 4 HotBot Edition instruments that feature patented contact printing technology installed in more than 4,200 laboratories worldwide. The Warranty Option is purchased in one-year increments and provides comprehensive one-year coverage on all instrument components and accessories (printhead and pins excluded) when used and operated under normal conditions.

SPA4W

3,684
Purchase1

SpotBot® 4 Personal Microarrayer Accessories

Arrayit SpotBot® 4 Personal Microarrayer Accessories provide the market's most advanced personal microarrayer accessories and components featuring our patented contact printing technology installed in more than 4,200 laboratories worldwide. SpotBot® 4 Accessories include SpotBot® 4 Professional Printhead, wash station, L-bracket, humidity pen, humidification reservoir, de-humidification reservoir, peristaltic pump tubing set, megasonic transducer, megasonic transducer wire, custom software, Internet-free dongle, and other accessories associated with SpotBot® 4, SpotBot® 4 Protein and SpotBot® 4 HotBot Microarrayers. Accessory pricing based on the specific accessory purchased.

SPA4A

2,500
Purchase1

*Pricing is recommended US list price. International prices may be higher due to taxes, duties, import fees, tariffs and other expenses. Please consult your distributor for pricing in your region. Shipping and handling should be added to all purchases.

 

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