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Products - Protein Microarrays - Pathogen Antigen Microarrays

Staphylococcus aureus
Arrayit Pathogen Antigen Microarrays permit rapid detection of reactive serum antibodies against a wide range pathogenic microorganisms including
Bartonella henselae, Borrelia burgdorferi, Brucella melitensis, Burkholderia pseudomallei, Chlamydia muridarum, Chlamydia trachomatis, Coxiella, Francisella tularensis, Mycobacterium tuberculosis, Orthopoxvirus Whole Proteome, Plasmodium falciparum, Plasmodium vivax, Salmonella enterica non-typhoidal, Salmonella enterica typhi, Staphylococcus aureus, and Toxoplasma gondii. Available as printed antigen microarrays and complete kits, this product line sets a new standard for research into the molecular basis of microbial infection.

Introduction
Arrayit Pathogen Antigen Microarray kits are available in 80, 160, 320 and 800 microarray packages sufficient for 80, 160, 320 and 800 serum screening assays respectively. These are highly multiplexed devices for measuring serum reactive antibodies to these pathogens. Each microarray chip contains key proteins proteins from each proteome plus an additional proteins as controls. The proteins on these microarrays are expressed by in vitro transcription/translation using an E. coli cell free expression system and printed onto nitrocellulose microarray substrate slides with each slide containing 3, 8 or 16 separate pads depending on the number of proteins for that organism. These protein microarrays have been validated by probing with serum specimen collections of disease cases and controls.

Antigen-Discovery-Substrates-690

Materials supplied

  1. Antigen Microarrays
  2. Blocking Buffer
  3. 20X Wash Buffer
  4. 20X TBS
  5. Secondary: biotin conjugated goat anti-bovine IgG
  6. Tertiary: streptavidin conjugated fluorophore
  7. Microarray Frame, Chambers and Clips

Note:  Microarray frames, chambers and clips are reusable.  Additional frames, chambers and clips can be purchased from GraceBio (cat# 246870, 246853 and 204838 respectively) or other vendors who can provide equivalent assemblies.

Additional Materials Required

  • Ultrapure water
  • Orbital shaker or rocker platform
  • Table top centrifuge
  • Any microarray slide scanner with a red (Cy5) laser, available from e.g. Molecular Devices LLC, Arrayit Corporation, Agilent Technologies, and other manufacturers.
  • Pipettors and tips
  • Disposable polypropylene or polystyrene tubes for sample preparation
  • Disposable dispensing reservoirs
  • Containers for wash buffer dilution
  • Timer
  • Aspirator: can be assembled from a vacuum, Erlenmeyer flask, tubing, and pipette tips

Storage and Handling

  • This product is shipped on cold pack or wet ice.  Immediately upon receipt, store the Seconday, Tertiary, and E. coli lysate tubes at -20°C.  All other components can be stored at room temperature.
  • The kit is stable until the expiration date indicated on the label when stored as specified.
  • The microarray slides are provided in a sealed pouch with desiccant. Once opened, any unused portion should be reclosed with desiccant in the pouch and returned to 4°C storage immediately.
  • Nitrocellulose pads should not be in direct contact with any solid object, e.g. a pipette tip, as they are susceptible to scratching.
  • Allow reagents to warm up to room temperature before use.
  • Do not use any reagent beyond the expiration date.
  • The diluted 1X Wash Buffer is stable the day of the preparation only.
  • Avoid exposure of fluorophore to light, air, or extreme temperatures.

Precautions

  • Read the manual before starting work.
  • Human serum is potentially pathogenic and should be handled in a class II biological containment hood. Wear appropriate PPE (disposable gloves, lab coat) when handling serum. Avoid aerosols and remove sharps from the work area. All liquid waste containing human serum should be inactivated with 10% (final concentration) chlorine bleach or equivalent.
  • All kit reagents should be handled according to good laboratory practice. Wear appropriate PPE (disposable gloves, lab coat). Use a dedicated workbench. No eating, drinking, applying cosmetics, smoking etc in the area. Follow laboratory guidelines for liquid and solid waste disposal or consult your biological safety officer.

Specimen collection and preparation

  • Collect serum or plasma specimens.  Serum should be separated from red blood cells as soon as possible.
  • If storing specimens, avoid repeated freezing and thawing.

Reagent Preparation

  • Reconstitute each lyophilized E.coli lysate (ECL) tube with 1.2 mL blocking buffer. Surplus lysate can be stored at -20°C.
  • Dilute wash buffer and TBS to 1X concentration.  Diluted buffers are only stable the day of preparation.  Be sure to only dilute the amount of buffer needed.

Procedure

 DAY 1:

  • If frozen, thaw serum samples on ice
  • Assemble the microarray frame, chamber and slide.  Cover with a lid.  Assembly of a 8-pad slide is shown below; the 3-pad slide contained in this kit is assembled in an identical manner.

Figure 1.  Frame, Chamber, clips and 8-pad slide before assembly

  • Figure 2.  Align the 8-pad slide and the chamber
  • Figure 3.  Snap one clip over one edge of the 8-pad slide/chamber
  • Figure 4.  Snap a second clip over the other edge of the 8-pad slide/chamber
  • Figure 5.  Place the completed 8-pad slide/chamber/clip into the frame
  • Figure 6.  Finished assembly (up to 3 slides can be assembled into the frame)
  • Block the microarrays with Provided Blocking Buffer per well for 30-60 minutes.
  • Make 10% ECL in Blocking Buffer for pre-incubation of serum samples. Now referred to as pre-incubation solution. 
  • In a Biosafety Cabinet, dilute serum 1 to 100 in pre-incubation solution and incubate for at least 30 minutes (350 µl/well needed for a 3-pad).
  • In a Biosafety Cabinet, remove blocking buffer from pads and immediately add diluted serum samples. Do not let the pads dry out.  Put the frames into a humidified airtight container.  Incubate overnight at 4 degrees with gentle agitation.

DAY 2:

  • Wash with 1mL 1X wash buffer per well 3 times for 5 minutes each.  Wash by aspirating the serum solution and immediately adding 1 mL 1X wash buffer, being careful not to let the pads dry out.
  • Make 1% ECL in Blocking Buffer for dilution of secondary antibody (Biotin conjugated goat anti-bovine IgG Fcγ) and Streptavidin conjugated fluor.  Now referred to as blocking solution.
  • Dilute secondary antibody 1 to 1,000 in blocking solution (350 µl/well needed for a 3-pad).
  • Remove wash buffer and add 350 µl diluted secondary antibody per well.
  • Incubate for 1 hour at room temperature.
  • Wash with 1 mL 1X wash buffer per well 3 times for 5 minutes each.
  • Dilute tertiary detection fluor 1 to 200 in blocking solution (350 µl/well needed for a 3-pad).  Protect from light until ready to use.
  • Remove wash buffer and add 350 µl diluted tertiary detection fluor per well.
  • Incubate for 1 hour at room temperature in the dark (i.e. cover with aluminum foil).
  • Wash with 1 mL 1X wash buffer per well 3 times for 5 minutes each.
  • Wash with 1 mL 1X TBS per well 3 times for 1 minute each.
  • Fill a 2L beaker with ultra pure H2O.
  • Remove slide from Fast frame assembly and dip in ultra pure water. Place into a 50 ml conical with a Kimwipe at the bottom. Spin at room temperature for 5 minutes at 500X g to dry.
  • Scan slides, quantify and analyze data.
  • *Note:  All incubation and washes are done on a rocking platform.  First washes after incubation should be more thorough, using an aspirator, but subsequent washes may discard solution by dumping as long as wash buffer can be added quickly to prevent drying of the pads.

Quality control

    Each microarray contains positive and negative control spots.

antigen-microarray

Data Analysis

  • Customers may choose their desired methods and software to perform data analysis.
  • If desired, data analysis service may be arranged with ADi upon request.

TB-microarray-data

Alternative Detection Methods

  • The Biotin-Streptavidin fluorophore detection system provided in this kit has demonstrated the best sensitivity throughout our extensive research and development.  However, customers may choose alternative procedures to detect the reactive antibodies in the human serum sample to suit their specific needs.  These include alkaline phosphatase conjugated secondary antibody with a coloarimetric detection system and fluorophore conjugated secondary antibody.
  • Furthermore, customers may detect the reactive antibodies in serum obtained from other animals by substituting a secondary antibody against the subject animal.  For instance, if the customer is interested in detecting antibody in bovine serum samples, biotin conjugated goat anti-bovine IgG Fcr (which can be purchased from many sources) would be substituted for the human secondary antibody contained in this kit.

Ordering Information

Product

Cat. ID

Price (US Dollars)*

B. henselae full proteome microarray

17MA0001

Call for quote

B. henselae full proteome microarray kit

17MA001K

Call for quote

B. henselae reactive antigen microarray, 80 microarrays

17MA0002

$4,000

B. henselae reactive antigen microarray, 80 microarray kit

17MA002K

$4,900

B. henselae reactive antigen microarray, 160 microarrays

17MA0003

$6,400

B. henselae reactive antigen microarray, 160 microarray kit

17MA003K

$7,300

B. henselae reactive antigen microarray, 320 microarrays

17MA0004

$11,200

B. henselae reactive antigen microarray, 320 microarray kit

17MA004K

$12,400

B. henselae reactive antigen microarray, 800 microarrays

17MA0005

$22,400

B. henselae reactive antigen microarray, 800 microarray kit

17MA005K

$24,200

B. burgdorferi ELISA kit

11EG001K

$250

B. burgdorferi microarray full proteome

11MA0001

Call for quote

B. burgdorferi microarray full proteome kit

11MA001K

Call for quote

B. burgdorferi reactive antigen microarray, 32 microarray kit

11MA010K

$2,900

B. burgdorferi reactive antigen microarray, 80 microarrays

11MA0002

$4,000

B. burgdorferi reactive antigen microarray, 80 microarray kit

11MA002K

$4,900

B. burgdorferi reactive antigen microarray, 160 microarrays

11MA0003

$6,400

B. burgdorferi reactive antigen microarray, 160 microarray kit

11MA003K

$7,300

B. burgdorferi reactive antigen microarray, 320 microarrays

11MA0004

$11,200

B. burgdorferi reactive antigen microarray, 320 microarray kit

11MA004K

$12,400

B. burgdorferi reactive antigen microarray, 800 microarrays

11MA0005

$22,400

B. burgdorferi reactive antigen microarray, 800 microarray kit

11MA005K

$24,200

B. burgdorferi AntigenStrip kit

11SG001K

$400

B. melitensis ELISA kit

12EG001K

$250

B. melitensis microarray  full proteome

12MA0001

Call for quote

B. melitensis microarray  full proteome kit

12MA001K

Call for quote

B. melitensis reactive antigen microarray, 32 microarray kit

12MA010K

$2,900

B. melitensis reactive antigen microarray, 80 microarrays

12MA0002

$4,000

B. melitensis reactive antigen microarray, 80 microarray kit

12MA002K

$4,900

B. melitensis reactive antigen microarray, 160 microarrays

12MA0003

$6,400

B. melitensis reactive antigen microarray, 160 microarray kit

12MA003K

$7,300

B. melitensis reactive antigen microarray, 320 microarrays

12MA0004

$11,200

B. melitensis reactive antigen microarray, 320 microarray kit

12MA004K

$12,400

B. melitensis reactive antigen microarray, 800 microarrays

12MA0005

$22,400

B. melitensis reactive antigen microarray, 800 microarray kit

12MA005K

$24,200

B. melitensis AntigenStrip kit

12SG001K

$400

B. pseudomallei full proteome microarray

23MA0001

Call for quote

B. pseudomallei full proteome microarray kit

23MA001K

Call for quote

B. pseudomallei reactive antigen microarray, 80 microarrays

23MA0002

$4,000

B. pseudomallei reactive antigen microarray, 80 microarray kit

23MA002K

$4,900

B. pseudomallei reactive antigen microarray, 160 microarrays

23MA0003

$6,400

B. pseudomallei reactive antigen microarray, 160 microarray kit

23MA003K

$7,300

B. pseudomallei reactive antigen microarray, 320 microarrays

23MA0004

$11,200

B. pseudomallei reactive antigen microarray, 320 microarray kit

23MA004K

$12,400

B. pseudomallei reactive antigen microarray, 800 microarrays

23MA0005

$22,400

B. pseudomallei reactive antigen microarray, 800 microarray kit

23MA005K

$24,200

C. muridarum full proteome microarray

26MA0001

Call for quote

C. muridarum full proteome microarray kit

26MA001K

Call for quote

C. muridarum reactive antigen microarray, 80 microarrays

26MA0002

$4,000

C. muridarum reactive antigen microarray, 80 microarray kit

26MA002K

$4,900

C. muridarum reactive antigen microarray, 160 microarrays

26MA0003

$6,400

C. muridarum reactive antigen microarray, 160 microarray kit

26MA003K

$7,300

C. muridarum reactive antigen microarray, 320 microarrays

26MA0004

$11,200

C. muridarum reactive antigen microarray, 320 microarray kit

26MA004K

$12,400

C. muridarum reactive antigen microarray, 800 microarrays

26MA0005

$22,400

C. muridarum reactive antigen microarray, 800 microarray kit

26MA005K

$24,200

C. trachomatis full proteome microarray

18MA0001

Call for quote

C. trachomatis full proteome microarray kit

18MA001K

Call for quote

C. trachomatis reactive antigen microarray, 80 microarrays

18MA0002

$4,000

C. trachomatis reactive antigen microarray, 80 microarray kit

18MA002K

$4,900

C. trachomatis reactive antigen microarray, 160 microarrays

18MA0003

$6,400

C. trachomatis reactive antigen microarray, 160 microarray kit

18MA003K

$7,300

C. trachomatis reactive antigen microarray, 320 microarrays

18MA0004

$11,200

C. trachomatis reactive antigen microarray, 320 microarray kit

18MA004K

$12,400

C. trachomatis reactive antigen microarray, 800 microarrays

18MA0005

$22,400

C. trachomatis reactive antigen microarray, 800 microarray kit

18MA005K

$24,200

C. burnetii full proteome microarray

16MA0001

Call for quote

C. burnetii full proteome microarray kit

16MA001K

Call for quote

C. burnetii reactive antigen microarray, 80 microarrays

16MA0002

$4,000

C. burnetii reactive antigen microarray, 80 microarray kit

16MA002K

$4,900

C. burnetii reactive antigen microarray, 160 microarrays

16MA0003

$6,400

C. burnetii reactive antigen microarray, 160 microarray kit

16MA003K

$7,300

C. burnetii reactive antigen microarray, 320 microarrays

16MA0004

$11,200

C. burnetii reactive antigen microarray, 320 microarray kit

16MA004K

$12,400

C. burnetii reactive antigen microarray, 800 microarrays

16MA0005

$22,400

C. burnetii reactive antigen microarray, 800 microarray kit

16MA005K

$24,200

F. tularensis full proteome microarray

22MA0001

Call for quote

F. tularensis full proteome microarray kit

22MA001K

Call for quote

F. tularensis reactive antigen microarray, 80 microarrays

22MA0002

$4,000

F. tularensis reactive antigen microarray, 80 microarray kit

22MA002K

$4,900

F. tularensis reactive antigen microarray, 160 microarrays

22MA0003

$6,400

F. tularensis reactive antigen microarray, 160 microarray kit

22MA003K

$7,300

F. tularensis reactive antigen microarray, 320 microarrays

22MA0004

$11,200

F. tularensis reactive antigen microarray, 320 microarray kit

22MA004K

$12,400

F. tularensis reactive antigen microarray, 800 microarrays

22MA0005

$22,400

F. tularensis reactive antigen microarray, 800 microarray kit

22MA005K

$24,200

M. tuberculosis full proteome microarray

15MA0001

Call for quote

M. tuberculosis full proteome microarray kit

15MA001K

Call for quote

M. tuberculosis reactive antigen microarray, 32 microarray kit

15MA010K

$2,900

M. tuberculosis reactive antigen microarray, 80 microarrays

15MA0002

$4,000

M. tuberculosis reactive antigen microarray, 80 microarray kit

15MA002K

$4,900

M. tuberculosis reactive antigen microarray, 160 microarrays

15MA0003

$6,400

M. tuberculosis reactive antigen microarray, 160 microarray kit

15MA003K

$7,300

M. tuberculosis reactive antigen microarray, 320 microarrays

15MA0004

$11,200

M. tuberculosis reactive antigen microarray, 320 microarray kit

15MA004K

$12,400

M. tuberculosis reactive antigen microarray, 800 microarrays

15MA0005

$22,400

M. tuberculosis reactive antigen microarray, 800 microarray kit

15MA005K

$24,200

Orthopoxvirus ELISA kit (DryVax)

13EG001K

$250

Orthopoxvirus ELISA kit (MVA)

13EG002K

$250

Orthopoxvirus full proteome microarray, 32 microarray kit

13MA010K

$2,900

Orthopoxvirus full proteome microarray, 80 microarrays

13MA0001

$4,000

Orthopoxvirus full proteome microarray, 80 microarray kit

13MA001K

$4,900

Orthopoxvirus full proteome microarray, 160 microarrays

13MA0002

$6,400

Orthopoxvirus full proteome microarray, 160 microarray kit

13MA002K

$7,300

Orthopoxvirus full proteome microarray, 320 microarrays

13MA0003

$11,200

Orthopoxvirus full proteome microarray, 320 microarrays

13MA003K

$12,400

Orthopoxvirus full proteome microarray, 800 microarrays

13MA0004

$22,400

Orthopoxvirus full proteome microarray, 800 microarrays

13MA004K

$24,200

Orthopoxvirus AntigenStrip kit (DryVax)

13SG001K

$400

Orthopoxvirus AntigenStrip kit (MVA)

13SG002K

$400

P. falciparum full proteome microarray

25MA0001

Call for quote

P. falciparum full proteome microarray kit

25MA001K

Call for quote

P. falciparum reactive antigen microarray, 16 microarray kit

25MA010K

$2,500

P. falciparum reactive antigen microarray, 40 microarrays

25MA0006

$4,000

P. falciparum reactive antigen microarray, 40 microarray kit

25MA006K

$4,500

P. falciparum reactive antigen microarray, 80 microarrays

25MA0007

$7,040

P. falciparum reactive antigen microarray, 80 microarray kit

25MA007K

$7,540

P. falciparum reactive antigen microarray, 160 microarrays

25MA0008

$12,800

P. falciparum reactive antigen microarray, 160 microarray kit

25MA008K

$13,550

P. falciparum reactive antigen microarray, 400 microarrays

25MA0009

$30,000

P. falciparum reactive antigen microarray, 400 microarray kit

25MA009K

$31,200

P. vivax full proteome microarray

24MA0001

Call for quote

P. vivax full proteome microarray kit

24MA001K

Call for quote

P. vivax reactive antigen microarray, 40 microarrays

24MA0006

$4,000

P. vivax reactive antigen microarray, 40 microarray kit

24MA006K

$4,500

P. vivax reactive antigen microarray, 80 microarrays

24MA0007

$7,040

P. vivax reactive antigen microarray, 80 microarray kit

24MA007K

$7,540

P. vivax reactive antigen microarray, 160 microarrays

24MA0008

$12,800

P. vivax reactive antigen microarray, 160 microarray kit

24MA008K

$13,550

P. vivax reactive antigen microarray, 400 microarrays

24MA0009

$30,000

P. vivax reactive antigen microarray, 400 microarray kit

24MA009K

$31,200

S. enterica non-typhoidal full proteome microarray

20MA0001

Call for quote

S. enterica non-typhoidal full proteome microarray kit

20MA001K

Call for quote

S. enterica non-typhoidal reactive antigen microarray, 80 microarrays

20MA0002

$4,000

S. enterica non-typhoidal reactive antigen microarray, 80 microarray kit

20MA002K

$4,900

S. enterica non-typhoidal reactive antigen microarray, 160 microarrays

20MA0003

$6,400

S. enterica non-typhoidal reactive antigen microarray, 160 microarray kit

20MA003K

$7,300

S. enterica non-typhoidal reactive antigen microarray, 320 microarrays

20MA0004

$11,200

S. enterica non-typhoidal reactive antigen microarray, 320 microarray kit

20MA004K

$12,400

S. enterica non-typhoidal reactive antigen microarray, 800 microarrays

20MA0005

$22,400

S. enterica non-typhoidal reactive antigen microarray, 800 microarray kit

20MA005K

$24,200

S. enterica typhi full proteome microarray

19MA0001

Call for quote

S. enterica typhi full proteome microarray kit

19MA001K

Call for quote

S. enterica typhi reactive antigen microarray, 80 microarrays

19MA0002

$4,000

S. enterica typhi reactive antigen microarray, 80 microarray kit

19MA002K

$4,900

S. enterica typhi reactive antigen microarray, 160 microarrays

19MA0003

$6,400

S. enterica typhi reactive antigen microarray, 160 microarray kit

19MA003K

$7,300

S. enterica typhi reactive antigen microarray, 320 microarrays

19MA0004

$11,200

S. enterica typhi reactive antigen microarray, 320 microarray kit

19MA004K

$12,400

S. enterica typhi reactive antigen microarray, 800 microarrays

19MA0005

$22,400

S. enterica typhi reactive antigen microarray, 800 microarray kit

19MA005K

$24,200

S. aureus microarray full proteome

14MA0001

Call for quote

S. aureus microarray full proteome kit

14MA001K

Call for quote

S. aureus reactive antigen microarray, 80 microarrays

14MA0002

$4,000

S. aureus reactive antigen microarray, 80 microarray kit

14MA002K

$4,900

S. aureus reactive antigen microarray, 160 microarrays

14MA0003

$6,400

S. aureus reactive antigen microarray, 160 microarray kit

14MA003K

$7,300

S. aureaus reactive antigen microarray, 320 microarrays

14MA0004

$11,200

S. aureaus reactive antigen microarray, 320 microarray kit

14MA004K

$12,400

S. aureus reactive antigen microarray, 800 microarrays

14MA0005

$22,400

S. aureus reactive antigen microarray, 800 microarray kit

14MA005K

$24,200

T. gondii full proteome microarray

21MA0001

Call for quote

T. gondii full proteome microarray kit

21MA001K

Call for quote

T. gondii reactive antigen microarray, 80 microarrays

21MA0002

$4,000

T. gondii reactive antigen microarray, 80 microarray kit

21MA002K

$4,900

T. gondii reactive antigen microarray, 160 microarrays

21MA0003

$6,400

T. gondii reactive antigen microarray, 160 microarray kit

21MA003K

$7,300

T. gondii reactive antigen microarray, 320 microarrays

21MA0004

$11,200

T. gondii reactive antigen microarray, 320 microarray kit

21MA004K

$12,400

T. gondii reactive antigen microarray, 800 microarrays

21MA0005

$22,400

T. gondii reactive antigen microarray, 800 microarray kit

21MA005K

$24,200

For ordering information, technical assistance and shipping schedules please contact us by telephone 408-744-1331 or email arrayit@arrayit.com, or use the purchase buttons to order directly to the Arrayit Store.

*International pricing may vary by as much as 30% or more because import duties, stocking fees and technical support.

*Add shipping and handling to all orders.

 

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