SuperStreptavidin

Products - Microarray Substrates & Slides - SuperStreptavidin

ArrayIt® SuperStreptavidin Microarray Substrates contain a highly uniform streptavidin treatment covalently bound to an atomically flat glass substrate, affording a universal high-capacity biotin-binding surface for DNA and protein microarray applications and many other life sciences applications that require a biotin binding surface. The open platform 25 x 76 mm glass substrate slide format renders SuperStreptavidin Microarray Substrates compatible with all standard microarrayers and scanners.

Table of Contents

Introduction
Quality Control
Product Description
Technical Specifications
Technical Assistance
Recommended Equipment
Ordering Information
Storage Conditions
Warranty

Introduction
Congratulations on taking a big step towards improving the affordability, quality and speed of your genomics, biomedical, pharmaceutical and agricultural research. This booklet contains all the information required to take full advantage of ArrayIt^® SuperStreptAvidin Microarray Substrates.

Quality Control
TeleChem takes every measure to assure the quality of our ArrayIt^® Brand Super Microarray Substrates. The finest microarray biochip cleanroom research was used to develop these products. Rigorous quality control monitoring on a substrate-by-substrate basis guarantees that each these products conforms to the highest industry standards.

Product Description
This surface starts with ArrayIt SuperClean glass then it is activated with streptavidin to bind biotinylated biomolecules. Streptavidin is protein isolated from streptomycetes avidinii that has an irreversible binding affinity for biotin. The streptavidin is covalently bound to the surface and will not flake, crack or peel off. The surface as a low intrinsic background and has a high binding capacity. SuperClean is a highly polished glass surface with an optical flatness tolerance of 50 angstroms over the entire 25mm x 76 mm surface area for optimum homogeneity; this new surface sets the highest quality standard for streptavidin-activated substrates for microarray applications. These substrates are packed in white slide boxes, vacuum packed and should be stored at 4 C.

Short Protocol Protein (Steps 1-7):
1. Suspend biotinylated protein samples in Protein Printing Buffer at 0.25-1 µg/µl.
2. Print protein samples onto SuperStrepavidin Substrates.
3. Block with SuperStreptavidin blocking buffer and then wash and dry the printed microarrays.
4. React the processed microarrays with fluorescent samples.
5. Wash the microarrays to remove un-reacted fluorescent material.
6. Scan the microarray to produce a fluorescent image.
7. Quantitate and model the fluorescent data.

Short Protocol DNA (Steps 1-7):
1. Suspend biotinylated DNA samples in Micro Spotting Solution Plus at 50uM final concentration.
2. Print DNA samples onto SuperStrepavidin Substrates.
3. Block with SuperStreptavidin blocking buffer and then wash and dry the printed microarrays.
4. Hybridize the processed microarrays with fluorescent probe.
5. Wash the microarrays to remove unreacted fluorescent probe.
6. Scan the microarray to produce a fluorescent image.
7. Quantitate and model the fluorescent data.

Technical Specifications:
SuperStreptavidin Microarray Substrates are not streptavidin-treated microscope slides! Atomically flat polished glass is used as the starting point for this product. Glass substrates of this quality routinely retail at $50-100 each from leading optical suppliers. Users will benefit from the following technical features:

Glass polished to atomic flatness (±20 angstroms)
Only polished glass surface in the microarray industry
Polishing improves topology and uniformity – keys to precise data
Homogenous streptavidin proteins provide superior biotin binding
Streptavidin molecules bound covalently to the glass substrate
Streptavidin monolayer contains 1.1 x 10¹⁰ streptavidin proteins/mm²
Vastly superior to unpolished optical quality glass from other vendors
Ultimate streptavidin surface for high density microarray applications
Ideal for contact and non-contact printing
Used by high-volume microarray core facilities
Manufactured in a state-of-the-art class 100 cleanrooms
Ultra-low intrinsic fluorescence and background noise
Open platform dimensions compatible with all major brands of microarrayers and scanners
Precise physical dimensions (25 ± 0.2 mm x 76 ± 0.3 mm x 0.940 mm ± 0.025 mm)
Proprietary corner chamfer (1.4 mm) provides unambiguous side and end orientation to simplify printing and processing
Finished edges enhance user safety
Excellent refractive index, transmission and hardness specifications
Sophisticated anti-static packaging improves usability
Offered with or without bar-codes
Custom laser and chrome fiducials available upon request
High-volume 100,000 piece per month manufacturing capabilities
Product arrives “ready to use” with no additional processing required

Figure 2. Atomic Force Microscopy (AFM) analysis. Shown are AFM scans of the SuperClean Microarray Substrate by TeleChem ArrayIt Division, Sunnyvale CA. Data are coded to a rainbow intensity scale, such that red data represents 5.0 nm or 50 angstroms. The substrates have an average flatness of 2.0 nm or 20 angstroms, which corresponds to about 10 silicon dioxide bonds.

Figure 3. Correct Substrate Orientation. Shown is a graphic of two ArrayIt® Microarray Substrates, showing the correct and incorrect orientation for use. In the correct orientation (blue graphic), the chamfer will be located in the upper right corner and samples should be printed on the side facing upward, which is the same side that contains the word “Correct!”. In the incorrect orientation (red graphic), the chamfer will be located in the upper left corner, placing the backside facing upward, which is the side that contains the word “Incorrect!”. Only one side of ArrayIt® Microarray Substrates is suitable for printing. Please print on the correct side only.

Figure 4. SuperStreptavidin reactive area is 24mm x 60mm as shown in the blue area on the above graphic.

Technical Assistance
Please contact us if you have any comments, suggestions, or if you need technical assistance. By electronic mail: arrayit@arrayit.com (under the subject heading please type "ArrayIt® technical assistance"). By telephone: (408) 744-1331, Monday–Friday PST 9:00am - 4:30pm. Please remember that we want to hear about your successes!

Scientific Publications
Click here and here for recent scientific publications using ArrayIt® brand microarray products from TeleChem International, Inc for streptavidin experimentation.

Ordering Information

Product

Description

Pieces per box

Catalog ID

Price (U.S. dollars)

SuperStreptavidin Substrates

Most advanced microarray substrate slide on the market, polished atomically flat glass with a covalently bound streptavidin layer for ultra-high biotin binding capacity and low non-specific binding over a 24 x 60 mm reactive area, 25 x 76 mm.

25

SMS

$420

SuperStreptavidin Substrates Barcoded

Most advanced microarray substrate slide on the market, polished atomically flat glass with a covalently bound streptavidin layer for ultra-high biotin binding capacity and low non-specific binding over a 24 x 60 mm reactive area, 25 x 76 mm with barcode.

25

SMSBC

$440

SuperStreptavidin Substrates Mirrored

Most advanced microarray substrate slide on the market, polished atomically flat glass with a covalently bound streptavidin layer for ultra-high biotin binding capacity and low non-specific binding over a 24 x 60 mm reactive area, reflective backside surface coating for 2-10X increased signal, 25 x 76 mm.

25

MRS

$920

SuperStreptavidin Substrates Mirrored and Barcoded

Most advanced microarray substrate slide on the market, polished atomically flat glass with a covalently bound streptavidin layer for ultra-high biotin binding capacity and low non-specific binding over a 24 x 60 mm reactive area, reflective backside surface coating for 2-10X increased signal, 25 x 76 mm with barcode.

25

MRSBC

$940

SuperStreptavidin Blocking Buffer

Advanced protein and small molecule blocking buffer that blocks and inactivates SuperStreptavidin microarray substrate slides prior to staining, greatly reduces background for all applications, 250 ml of 1X buffer solution.

NA

SBB

$250

Substrates Discounts:
4-19 boxes, 5% discount off list price
20-39 boxes, 10% discount off list price
40-199 boxes, 15% discount off list price

Blocking Buffer Discounts:
2-5 bottles, 5% discount off list price ($238)
6-10 bottles, 10% discount off list price ($225)
11-20 bottles, 15% discount off list price ($213)
20+ bottles, 20% discount off list price ($200)

*Plus shipping and handling via Federal Express or US Postal Service Priority Mail
International pricing may vary as much as 20% due to import duties, stocking fees and technical support. To order ArrayIt® Brand Products: call 408-744-1331, fax 408-744-1711, email arrayit@arrayit.com, or click on the Purchase buttons to proceed directly to the shop site.

Storage Conditions
This surface is manufactured and packaged in state-of-the-art class 100-cleanroom facilities. Store at 4°C and use within 2 months from the date of receipt. Store in the sealed plastic shipping envelope and avoid exposure to elevated temperatures and dust.

Warranty
TeleChem's Super Microarray Substrate products have been scientifically developed and manufactured in state-of-the-art class 100 cleanrooms. All of these products are sold for research purposes. Extreme care and exact attention should be practiced in the use of the materials described herein. All TeleChem products are subject to extensive quality control and are guaranteed to perform as described when used properly. Any problems with these products should be reported to TeleChem immediately. Our liability is limited to the replacement of the product, or a full refund. Any misuse of this product is the full responsibility of the user, and TeleChem makes no warranty or guarantee under such circumstances.