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Services - Peptide Microarray Manufacturing
Custom Peptide Microarray Manufacturing Services from Arrayit Corporation provide unprecedented access to high quality peptide microarrays at an unbeatable price. Every microarray is manufactured in one of our state-of-the-art class 100 clean room facilities, with the most advanced robotics, surfaces and coupling chemistries in the industry. Our particle, protease and ribonuclease free manufacturing laboratories provide microarrays of dazzling quality.
To receive a quotation e-mail us the peptide sequence information you desire to have on your microarray, the number of replicates for each peptide, and the number of microarrays desired to arrayit@arrayit.com.
Arrayit Peptide Microarray FAQs
Microarrays are compatible with all standard slide based fluorescent microarray scanners, quantification software and processing tools.
Thousands of microarrays can be manufactured with a single peptide library synthesis
Arrayit can provide technical assistance and all tools necessary for processing, scanning and quantifying the microarrays. We can also empower end users to manufacture their own peptide microarrays.
Peptides are covalently bound to a glass slide microarray substrate and available for a wide variety of binding assays including:
- biomarker discovery
- epitope mapping
- immunoassays
- protein interactions
- kinase screening
- protease screening
- phosphatase screening
- affinity profiling
- protein fingerprinting
Arrayit Peptide Microarrays are stable for up to 1 year at room temperature.
Arrayit’s patented microarray manufacturing technology empowers from 10 to tens of thousands of peptides per microarray with extremely low CV’s. Multiple microarrays per slide substrate can also be provided.
Ex-Situ 100% mass spec QC on each peptide before microarrays are manufactured
Microarray format provides very low reaction volumes, from 10 to 500 micro-liters.
Peptide Microarrays can be manufactured with any number of technical replicates and controls.
Peptide microarrays are compatible with standard slide based microarray scanners. For best results consider Innoscan 710 Microarray Scanner.
Compatible with standard ELISA protocols, formats can be provided to be compatible with ELISA processing automation.
Figure 1. Shows that the FLAG peptides epitopes were recognized by the ANTI-FLAG antibodies, but not by the ANTI-CD28 antibody used as an isotype matched control (negative control). In addition, the scrambled FLAG sequence that was used as another negative control was also not recognized by the ANTI-FLAG antibodies. These results indicate the specificity of the peptide-antibody binding. Figure 1 also shows that peptides containing additional sequences from both PILL and GST demonstrated enhanced reactivity with the ANTI-FLAG antibody. Adding PILL or GST sequences to the peptides did not enhance reactivity to the irrelevant antibody. Data courtesy of Dr. Jonathan Graf and Dr. John Imboden, UCSF. Peptides courtesy of Sigma-Aldrich.
Arrayit’s peptide microarray capabilities are well documented, e-mail us for a list of publications arrayit@arrayit.com
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