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Services - Peptide Microarray Manufacturing
Custom Peptide Microarray Manufacturing Services from Arrayit Corporation provide unprecedented access to high quality peptide microarrays at an unbeatable price. Every microarray is manufactured in one of our state-of-the-art class 100 clean room facilities, with the most advanced robotics, surfaces and coupling chemistries in the industry. Our particle, protease and ribonuclease free manufacturing laboratories provide microarrays of dazzling quality.
To receive a quotation e-mail us the peptide sequence information you desire to have on your microarray, how you would like the peptides constructed (example :20mer peptides overlapping by 15 amino acids) the number of replicates for each peptide, and the number of microarrays desired to arrayit@arrayit.com. 1 or more microarrays per slide substrate can be provided depending on the size of the library. Mutliplexed formats are available where up to 24 individual identical microarrays are printed onto a single slide substrate and can be processed in a 96 or 24 well format. See mutiplex and multi-well reaction tools at:
http://arrayit.com/Products/Microarray_Tools/microarray_tools.html
Arrayit Peptide Microarray FAQs
Microarrays are compatible with all standard slide based fluorescent microarray scanners, quantification software and processing tools.
Microarray are manufacturing using Arrayit’s patented technology, US Patent 6,101,946.
Thousands of microarrays can be manufactured with a single peptide library synthesis
Arrayit can provide technical assistance and all tools necessary for processing, scanning and quantifying the microarrays. We can also empower end users to manufacture their own peptide microarrays using the SpotBot and NanoPrint Microarrayers.
Peptides are covalently bound to a glass slide microarray substrate and available for a wide variety of binding assays including:
- biomarker discovery
- antibody profiling
- epitope mapping
- immunoassays
- protein interactions
- kinase screening
- protease screening
- phosphatase screening
- affinity profiling
- protein fingerprinting
Arrayit Peptide Microarrays are stable for up to 1 year at room temperature.
Peptide sequences on scan be as long as 20 amino acids.
Arrayit’s patented microarray manufacturing technology empowers from 10 to tens of thousands of peptides per microarray with extremely low CV’s. Multiple microarrays per slide substrate can also be provided.
Ex-Situ 100% mass spec QC on each peptide before microarrays are manufactured
Microarray format provides very low reaction volumes, from 10 to 500 micro-liters.
Peptide Microarrays can be manufactured with any number of technical replicates and controls.
Peptide microarrays are compatible with all standard slide based microarray scanners. For best results consider using the Innoscan 710 Microarray Scanner.
Compatible with standard ELISA protocols, formats can be provided to be compatible with ELISA processing automation. see:
http://arrayit.com/Products/Microarray_Tools/microarray_tools.html
Figure 1. Shows that the FLAG peptides epitopes were recognized by the ANTI-FLAG antibodies, but not by the ANTI-CD28 antibody used as an isotype matched control (negative control). In addition, the scrambled FLAG sequence that was used as another negative control was also not recognized by the ANTI-FLAG antibodies. These results indicate the specificity of the peptide-antibody binding. Figure 1 also shows that peptides containing additional sequences from both PILL and GST demonstrated enhanced reactivity with the ANTI-FLAG antibody. Adding PILL or GST sequences to the peptides did not enhance reactivity to the irrelevant antibody. Data courtesy of Dr. Jonathan Graf and Dr. John Imboden, UCSF. Peptides courtesy of Sigma-Aldrich. Peptides can be up to 20 amino acids in length.
Arrayit’s peptide microarray capabilities are well documented, e-mail us for a list of publications arrayit@arrayit.com
Applications for Peptide Microarrays
Identification of protein-protein interaction sites
Identifying protein-protein interaction sites is an extremely valuable tool enabling the design inhibitors, enhancers as well as providing information on binding regions of proteins
Epitope Mapping
Epitope mapping identifies antibody binding domains. These regions can be used in diagnostics or even implemented in vaccine for drug development.
Drug development
Drug developers want a molecule that specifically interacts with a target protein. Molecules designed to compete with or crowd out other proteins which would otherwise bind to the target, result in either blocking or enhancing protein function.
Diagnostics & Identification of Biomarkers
Peptide microarrays can diagnose disease. Peptides as biomarkers in a miniaturized and multiplexed format test can simultaneously screen for dozens to thousands of biomarkers in a single assay with an eaiser and less expensive protocol than DNA microarrays.
Peptide Library Synthesis Cost Prior to Microarray Manufacturing. We can help you with design, email us your sequence information. arrayit@arrayit.com
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Number of Peptides
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Description
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Price each
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Synthesis of 24 - 48 Peptides
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6-20mer peptides
0.5-2 mg guaranteed delivered (enough material to make thousands of microarrays)
100% Mass Spectral Analysis
N-terminal linker, C-terminus amide
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$60.00
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49 - 150 Peptides
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6-20mer peptides
0.5-2 mg guaranteed delivered (enough material to make thousands of microarrays)
100% Mass Spectral Analysis
N-terminal linker, C-terminus amide
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$55.00
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151 - 300 Peptides
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6-20mer peptides
0.5-2 mg guaranteed delivered (enough material to make thousands of microarrays)
100% Mass Spectral Analysis
N-terminal linker, C-terminus amide
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$49.00
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301 - 500 Peptides
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6-20mer peptides
0.5-2 mg guaranteed delivered (enough material to make thousands of microarrays)
100% Mass Spectral Analysis
N-terminal linker, C-terminus amide
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$44.00
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501 - 1000 Peptides
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6-20mer peptides
0.5-2 mg guaranteed delivered (enough material to make thousands of microarrays)
100% Mass Spectral Analysis
N-terminal linker, C-terminus amide
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$42.00
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>1000 Peptides
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6-20mer peptides
0.5-2 mg guaranteed delivered (enough material to make thousands of microarrays)
100% Mass Spectral Analysis
N-terminal linker, C-terminus amide
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$39.00
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Comon Formats, Number of Microarays per Slide:
Cost per Microarray is dependant on the number of microarrays ordered. Discounts available for voume orders. Contact us with your requirments arrayit@arrayit.com
Copyright 1993-2011 Arrayit Corporation. All rights reserved.
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