DNA Microarray CGH

Data Sheet

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Shown are the the phases of the comparative genomic hybridization (CGH) experimental lifecyle and how ArrayIt® products integrate into every phase of the CGH process. 1. High-end microarray robots equipped with the 946 Micro Spotting Device are used for CGH microarray manufacturing.  2. Depending on the experimental design, CGH microarrays can be made using BACs, oligonucleotides or cDNAs. Sample type influences surface chemistry. 3. Printed CGN microarrays are hybridized using Hybridization cassettes and other tools. 4. Detection for subsequent analysis in 2-colors is performed using a substrate slide based microarray scanner such as the SpotLight and Innoscan Microarray Scanners.


CGH-microarray
Shown are the the phases of the comparative genomic hybridization (CGH) experimental lifecyle and how ArrayIt® products integrate into every phase of the CGH process. 1. High-end microarray robots equipped with the 946 Micro Spotting Device are used for CGH microarray manufacturing.  2. Depending on the experimental design, CGH microarrays can be made using BACs, oligonucleotides or cDNAs. Sample type influences surface chemistry selection. 3. Printed CGN microarrays are hybridized using Hybridization cassettes and other tools. 4. Detection for subsequent analysis in 2-colors is performed using a substrate slide based microarray scanner such as the SpotLight and Innoscan Microarray Scanners.

Recommended Equipment and Reagents
946 and Stealth Micro Spotting Devices
These are the most widely used microarray technologies in the world, with many thousands of installations world wide on various robotic systems.  It is used to manufacture CGH microarrays of BACs, cDNAs or oligos.  It is compatible with all the various microarray surface chemistries deployed in CGH microarray applications.

NanoPrint Microarrayer
High throughput linear drive motion control based microarrayer capable of more than 48 spots per second, and 50,000 spots per microarray. This instrument sets the standard for CGH microarray manufacturing applications regardless of the sample type.

SpotBot® 3 Microarrayers
Low throughput microarrayer excellent for personal CGH microarray manufacturing.

SpotLight Microarray Scanner
The architecture of this 10-micron resolution, 2-color system increases signal to noise ratios in CGH microarray scans by 7X over traditional microarray scanning architectures.

InnoScan 700, 710, 900 and 910 Microarray Scanners
High Resolution 2-color scanner for both in-situ and ex-situ manufactured microarrays.

Hybridization Station
For both microarray and ISH slide processing applications.

Microarray slides (surface chemistries)
Depending on the sample type and preparation protocol, ArrayIt® has a surface chemistry to meet the needs of all CGH microarray applications.  BACs, cDNAs, oligos, DOP-PCR, Linker-mediated PCR-amplified  generated samples and many other types are compatible.  Tell us your sample type and we can recommend the right surface chemistry.

Hybridization Cassettes
This simple, yet high performance bench top tool delivers consistent hybridization results at very low cost.  Durable and lasting performance for many years is guaranteed.

High Throughput Wash Station
Simple and inexpensive tool to get stunning microarray results.

Microarray Centrifuge
This tool provides quick and efficient drying of CGH microarrays prior to scanning.

Purification Kits
Cleaner samples in microarray applications provide superior results.

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